Journal: The Journal of Experimental Medicine

Article Title: A Monoclonal Antibody to the α2 Domain of Murine Major Histocompatibility Complex Class I that Specifically Kills Activated Lymphocytes and Blocks Liver Damage in the Concanavalin A Hepatitis Model

doi: 10.1084/jem.20021301

Figure Lengend Snippet: Expression of RE2 epitope and cytotoxic sensitivity to mAb RE2 of the human HLA-A, B-negative EBV-transformed B cell line C1R, transfected with xeno-chimeric MHC class I genes composed of either murine H-2K b or human HLA-B7 at α1, α2, and α3 domains. Expression of RE2 molecules was analyzed by incubating on ice target cells with mAb RE2, followed by FITC-conjugated mouse anti–rat immunoglobulins, and cells were subjected to the analysis using FACStar PLUS™ . Cytotoxic sensitivity of transfectants to mAb RE2 was analyzed, as described in Materials and Methods (right column).

Article Snippet: Antibiotic-resistant clones were isolated and expanded, and expression of the hybrid MHC class I molecules was confirmed, using flow cytometric analysis with FACStar PLUSTM (Becton Dickinson).

Techniques: Expressing, Transformation Assay, Transfection

Journal: The Journal of Experimental Medicine

Article Title: A Monoclonal Antibody to the α2 Domain of Murine Major Histocompatibility Complex Class I that Specifically Kills Activated Lymphocytes and Blocks Liver Damage in the Concanavalin A Hepatitis Model

doi: 10.1084/jem.20021301

Figure Lengend Snippet: Therapeutic effects of mAb RE2 on Con A–induced hepatitis in mice. (A) Serum levels of GOT and GPT in C57BL/6 mice ( n = 6) 24 h after an intravenous injection of Con A, with or without administration of mAb RE2. Closed and open bars represent serum levels of GOT and GPT, respectively. (B) Histopathology of massive liver cell necrosis seen in a mouse 24 h after an intravenous injection of Con A. (C) Absence of Con A-induced liver cell necrosis in a mouse treated with mAb RE2. (D) Reduction of CD69-positive activated CD4 + and CD8 + T cells and NK1.1 + CD3 + NKT cells in the liver of mice given Con A and mAb RE2 (bottom), compared with findings in mice given Con A alone (top). NKT cells were examined by triple staining of cells with mAbs to CD69, NK1.1, and CD3, and gated CD3 + cells were analyzed for CD69 and NK1.1, using FACStar PLUS™ .

Article Snippet: Antibiotic-resistant clones were isolated and expanded, and expression of the hybrid MHC class I molecules was confirmed, using flow cytometric analysis with FACStar PLUSTM (Becton Dickinson).

Techniques: Injection, Histopathology, Staining